Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Product Availability Update

Promega Manufacturing and Delivery Systems continue to be fully operational during the COVID-19 outbreak. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Learn more

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Sign up for Promega updates and get 15% off your next Promega.com web order! Sign up >

HiBiT Protein Tagging System

Detect and quantify any tagged protein with a simple, luminescent signal.

HiBiT simplifies protein detection, providing a streamlined, antibody-free protocol for detecting tagged proteins using a convenient, bioluminescence based method. With the dynamic range to detect proteins without overexpression, the convenience of a single-reagent-addition method, and live cell detection options, HiBiT technology opens up a world of possibilities for researchers studying protein biology.

How to tag proteins with HiBiT

Choose from 3 HiBiT Detection Methods



Sensitive quantification of HiBiT-tagged proteins in cell lysates, IP complexes, or other cell-free systems.

Visit Product Page


Used with live cells to quantify surface-expressed or secreted proteins.

Visit Product Page


Detects any tagged protein on a blot in a few minutes with a simple luminescent signal.

Visit Product Page

Example Applications of HiBiT Technology

Tag Endogenous Proteins via CRISPR Insertion—No Cloning Required

HiBiT technology makes CRISPR–mediated tag knock-ins accessible by eliminating the need for molecular cloning prior to insertion and simplifying detection of tagged proteins. The small tag size makes CRISPR insertion efficient and the bioluminescence enables antibody-free detection with the sensitivity to detect endogenous expression.


Monitor Receptor Internalization

The HiBiT detection method eliminates the need for antibody-based methods for receptor internalization studies. With HiBiT technology, the multiple antibody binding steps and associated washes are eliminated from detection protocols. Simply add the detection reagent and measure a luminescent signal.


Quantify Protein Abundance and Degradation

Classic epitope tagging methods are limited in throughput or sensitivity, require high-quality antibodies and may only yield semi-quantitative results. HiBiT tagging brings the simplicity and sensitivity of bioluminescence to studies on protein abundance, quantifying proteins down to endogenous levels, even those maintained at low expression levels.


Speed and Simplify Western Blotting

The HiBiT blotting system is a sensitive and fast alternative to laborious Western blotting techniques that does not require an antibody.

大香伊在人线国产观看?大香伊在人线综合 大萫焦视频