Stable Luminescence Signal Supports Batch Processing
The ONE-Glo? EX Assay offers many of the same benefits of the ONE-Glo? Assay, including use of ?5′-fluoroluciferin substrate and an add-mix-read, or homogeneous, protocol. However, the ONE-Glo? EX Reagent uses a new assay chemistry to increase the stability of both the luminescence signal and the reconstituted reagent. The approximately 2-hour signal half-life provides greater flexibility in assay design.
The example data shown here illustrate how the ONE-Glo? EX signal persisted over 2 hours. In this experiment, 100μl purified firefly luciferase (13.8ng/ml) was combined in a 96-well plate with 100μl of Bright-Glo?, ONE-Glo?, ONE-Glo? EX, Dual-Glo? Luciferase or Steady-Glo? Reagents. Luminescence was measured periodically over 2 hours, n = 8.