Two-Step Reverse Transcription and qPCR
The GoTaq? Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript? Reverse Transcription System and GoTaq? Probe qPCR Master Mix.
The GoTaq? Probe qPCR Master Mix contains GoTaq? Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
The GoScript? Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.