Coronavirus SARS-CoV-2 Detection and Assay Development
The emergence of new viral diseases, including the coronavirus SARS-CoV-2 (COVID 19), MERS (2015) and SARS (2003) outbreaks, highlights the need for fast methods to detect and identify target viruses at scale. The COVID-19 pandemic also illustrates how research efforts to develop drug treatments or vaccines are accelerated in times of urgent need. The need for supplies and support for scientists working to curtail spread of the SARS-CoV-2 virus is urgent and immediate.
Promega supports scientists working to understand the molecular mechanisms by which emerging viruses infect humans and animals, and to develop accurate detection methods. We provide collaborative technical support, nucleic acid purification expertise, amplification products and labeling technologies that streamline workflows and expedite the work of research labs studying coronaviruses and other emerging viral diseases.
CDC COVID-19 Emergency Use Protocol Option
The GoTaq® Probe 1-Step RT-qPCR System is now a recommended master mix option for the Center for Disease Control and Prevention’s (CDC) 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel that is available through the FDA’s Emergency Use Authorization (EUA).
“Labs now have one more CDC-approved master mix when performing the 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel"
Amplification Products for Viral Analysis
qPCR and RT-qPCR
PCR reagents, including qPCR and RT-qPCR products are used to detect and amplify target viral sequences. These reagents are used widely in virology research studies. The GoTaq® Probe 1-Step RT-qPCR System is a recommended master mix option for the Center for Disease Control and Prevention’s (CDC) 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel.
PCR Reagents and Accessory Products
Diagne, M. et al. (2020) Dianke virus: A new mesonivirus species isolated from mosquitoes in Eastern Senegal. Virus Research 275, 2 January 2020, 197802.
Alves, M.T. et al. (2018) Mamastrovirus 5 detected in a crab-eating fox (Cerdocyon thous): Expanding wildlife host range of astroviruses. Comparative Immunology, Microbiology and Infectious Diseases 58, 36-43.
qPCR for Detection of Viral RNA:
Cai, M. et al. (2020) The R251K Substitution in Viral Protein PB2 Increases Viral Replication and Pathogenicity of Eurasian Avian-like H1N1 Swine Influenza Viruses. Viruses 12(1), 52.
Viral Structure and Characterization:
Wicht, O. et al. (2014) Proteolytic Activation of the Porcine Epidemic Diarrhea Coronavirus Spike Fusion Protein by Trypsin in Cell Culture. J. Virol. 88(14), 7952–7961.
Automated Viral Nucleic Acid Extraction
Maxwell® instruments are used together with Maxwell® Viral Total Nucleic Acid Purification Kits to extract viral nucleic acids from up to 48 samples per run.
Maxwell? RSC Viral Total Nucleic Acid Purification Kit
Viral total nucleic acid extraction from various samples types using the Maxwell? RSC Instrument. For Research Use Only.
Maxwell? 16 Viral Total Nucleic Acid Purification Kit
Extracts viral RNA and DNA from serum or plasma. Requires Maxwell? 16 Instrument.
High-Throughput Automation and Manual Purification Chemistries
ReliaPrep™ Viral TNA Miniprep Kit, Custom
The ReliaPrep™ Viral TNA Miniprep Kit, available as a custom order, uses spin columns for rapid centrifugation-based processing of viral samples. See the Application Notes provided below for example protocols.
Maxwell® HT Viral TNA Kit, Custom
The Maxwell® HT Viral TNA Kit uses magnetic resin for easy automation and separation using robotic liquid handlers. The kit can also be used for manual purification in multiwell plates. For example protocols using the Maxwell® HT Viral TNA Kit on Tecan, Hamilton, Eppendorf and Kingfisher robots, and for example manual purification protocols, please review the Application Notes provided below.
Please contact us for information on pricing and availability.
Purification Scale-Up and Automation Support
Promega Field Support Scientists (FSS) are available to help labs implement or optimize automation of viral RNA extraction methods, regardless of the instrumentation platform used.
If you are new to automation, pressed for time, or need help choosing and adapting a viral nucleic acid purification method, we can help. We provide chemistry and instrumentation expertise and will work with you to configure your instrument and optimize the workflow to meet your laboratory's needs.
Viral RNA Extraction Application Notes
ReliaPrep? Viral TNA Miniprep Kit, Custom
Maxwell? HT Viral TNA Kit
Maxwell? Instrument Purification
Viral RNA Extraction Using Maxwell? 48:
Jaeger, A.S. et al. (2018) Zika viruses of both African and Asian lineages cause fetal harm in a vertical transmission model. PLOS Neglected Tropical Diseases doi: 10.1371/journal. pntd.0007343
Chantal Reusken, C. et al. (2019) Toscana, West Nile, Usutu and tick-borne encephalitis viruses: external quality assessment for molecular detection of emerging neurotropic viruses in Europe, 2017. Euro Surveill. Dec 12; 24(50): 1900051.
Molecular assays using PCR have become the method of choice for viral detection due to their specificity and speed. This article discusses key considerations to take into account during the assay development process, including:
- Guidelines for decisions for extraction, amplification, and detection for assay design
- Links to reference materials, including 2019-COVID primer sequences
- Strategy for future virus detection assay development
“With proper planning and research, a laboratory can rapidly create a reproducible method for detection of a viral target.”
Evaluation of anti-viral drug candidates:
Zhang, L. et al. (2020) Alpha-ketoamides as broadspectrum inhibitors of coronavirus and enterovirus replication. Structure-based design, synthesis, and activity assessment. J. Med Chem. DOI: 10.1021/ acs.jmedchem.9b01828. Publication Date (Web): 11 Feb 2020
Sheahan, T.P et al. (2020) Comparative therapeutic efficacy of remdesivir and combination lopinavir, ritonavir, and interferon beta against MERS-CoV. Nature Communications 11, Article number: 222.
Testing for viral antibodies in serum using viral proteins fused to Nanoluc® luciferase:
Wang, W. et al. (2019) A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals. Biosafety and Health 1(3), 134-143.
Monitoring viral distribution by bioluminescence imaging:
Shen, L. et al. (2019) High-Throughput Screening and Identification of Potent Broad-Spectrum Inhibitors of Coronaviruses. DOI: 10.1128/ JVI.00023-19. J. Virol. 93(12), e00023-19.
Coronavirus SARS-CoV-2 News and Updates
Last updated April 28, 2020
SARS-CoV-2 is a novel coronavirus that emerged in late 2019 and is now the cause of a global pandemic. The virus presents a unique and acute challenge to researchers and public health officials. The news and articles below are updated regularly as the situation evolves. More information on how Promega manufacturing is responding to our customers needs during this time is available in this article.